N-（膦酰甲基）甘氨酸对分离的大鼠肝脏线粒体中能量依赖性转氢酶反应的抑制作用。

Inhibition of energy-dependent transhydrogenase reaction by N-)phosphonomethyl) glycine in isolated rat liver mitochondria.

作者信息

Olorunsogo O O

出版信息

Toxicol Lett. 1982 Jan;10(1):91-5. doi: 10.1016/0378-4274(82)90273-9.

Abstract

The pattern of interaction of various concentrations of N-phosphonomethyl derivative of glycine (PMG) with membrane-bound nicotinamide nucleotide transhydrogenase has been investigated in intact mitochondria isolated from rat liver. Lower concentrations of PMG ( less than 1.50 . 10(-4)M) had no significant effect (12% inhibition) on the activity of the enzyme when the reaction was supported by energy generated from succinate oxidation. Inhibition increased as the concentration of the herbicide was raised: at 3.12 . 10(-4) the degree was 28% and at 1.25 . 10(-3)M PMG, 46% (maximal inhibition). Similar results were obtained when ATP was used as the source of energy. These observations indicate that like thyroxine, an uncoupler of oxidative phosphorylation, PMG interacts with both oxidative phosphorylation and energy-dependent transhydrogenase reaction.

摘要

已对从大鼠肝脏分离出的完整线粒体中，不同浓度的甘氨酸N-膦酰甲基衍生物（PMG）与膜结合的烟酰胺核苷酸转氢酶的相互作用模式进行了研究。当反应由琥珀酸氧化产生的能量支持时，较低浓度的PMG（低于1.50×10⁻⁴M）对该酶的活性没有显著影响（抑制率为12%）。随着除草剂浓度的升高，抑制作用增强：在3.12×10⁻⁴时，抑制程度为28%，在1.25×10⁻³M PMG时，抑制率为46%（最大抑制）。当使用ATP作为能量来源时，也获得了类似的结果。这些观察结果表明，与氧化磷酸化解偶联剂甲状腺素一样，PMG与氧化磷酸化和能量依赖性转氢酶反应均相互作用。