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二环己基碳二亚胺和丁二酮对大鼠肝脏亚线粒体颗粒中烟酰胺核苷酸转氢酶的抑制作用。

Inhibition of nicotinamide nucleotide transhydrogenase in rat liver submitochondrial particles by dicyclohexylcarbodi-imide and butanedione.

作者信息

Moody A J, Reid R A

出版信息

Biochem J. 1983 Mar 1;209(3):889-92. doi: 10.1042/bj2090889.

DOI:10.1042/bj2090889
PMID:6870796
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1154171/
Abstract

Nicotinamide nucleotide transhydrogenase in rat liver submitochondrial particles is inhibited by treatment with NN'-dicyclohexylcarbodi-imide or butane-2,3-dione. Both inhibitions are pseudo-first-order with respect to enzyme activity. The reaction order with respect to inhibitor is close to unity for butanedione, but is significantly lower than unity for dicyclohexylcarbodi-imide.

摘要

大鼠肝脏亚线粒体颗粒中的烟酰胺核苷酸转氢酶可被用N，N'-二环己基碳二亚胺或丁二酮处理所抑制。两种抑制作用对于酶活性而言均为假一级反应。对于丁二酮，相对于抑制剂的反应级数接近1，但对于二环己基碳二亚胺则显著低于1。

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本文引用的文献

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Myokinase and adenosinetriphosphatase in oxidative phosphorylation.氧化磷酸化中的肌激酶和三磷酸腺苷酶
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Pyridine nucleotide transhydrogenase. VII. Determination of the reactions with coenzyme analogues in mammalian tissues.吡啶核苷酸转氢酶。VII。哺乳动物组织中与辅酶类似物反应的测定。
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J Biol Chem. 1981 Sep 10;256(17):8963-9.
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Inhibition of the mitochondrial nicotinamide nucleotide transhydrogenase by dicyclohexylcarbodiimide and diethylpyrocarbonate.二环己基碳二亚胺和焦碳酸二乙酯对线粒体烟酰胺核苷酸转氢酶的抑制作用。
J Biol Chem. 1981 Aug 10;256(15):8217-21.
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DCCD inhibits proton translocation and electron flow at the second site of the mitochondrial respiratory chain.二环己基碳二亚胺抑制线粒体呼吸链第二个位点的质子转运和电子流动。
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The properties of dicyclohexylcarbodiimide as an inhibitor of oxidative phosphorylation.二环己基碳二亚胺作为氧化磷酸化抑制剂的性质。
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