胞质蛋白在大鼠肝脏血红素细胞内转运中的作用。一种双标记方法。
The role of cytosolic proteins in the intracellular transport of haem in rat liver. A dual-label approach.
作者信息
Davies D M, Liem H H, Johnson E F, Muller-Eberhard U
出版信息
Biochem J. 1982 Jan 15;202(1):211-6. doi: 10.1042/bj2020211.
After consecutive injections of delta-amino[3H]- and -[14C]-laevulinic acid, the incorporation of the two labels into haem associated with different subfractions of the liver was determined. Marked differences in the 14C/3H ratios were observed between haem associated loosely and tightly with microsomes and mitochondria and haem associated with three subfractions of the cytosol obtained by gel filtration. The effect of changing the amounts of delta-aminolaevulinic acid injected and of changing the interval between injections and killing of the animal on the ratios of labels in the haem of each subfraction was studied. The results are discussed in terms of the flow of haem from the mitochondria to other parts of the cell via putative cytosolic carrier proteins.
连续注射δ-氨基[³H]-和-[¹⁴C]-戊酮酸后，测定了这两种标记物掺入肝脏不同亚组分中血红素的情况。观察到与微粒体和线粒体松散和紧密结合的血红素以及通过凝胶过滤获得的细胞溶质三个亚组分中血红素的¹⁴C/³H比值存在显著差异。研究了改变注射的δ-氨基戊酮酸量以及改变注射与处死动物之间的间隔对各亚组分血红素中标记物比值的影响。根据血红素通过假定的细胞溶质载体蛋白从线粒体流向细胞其他部位的情况对结果进行了讨论。
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