Wang J C, Tobin M S
Br J Haematol. 1982 Jun;51(2):277-84.
The saponin-induced myelofibrosis and myeloid metaplasia model in the rabbit was used to study mechanisms of extramedullary haematopoiesis. Haematopoietic progenitor cells, erythroid colony forming units (CFU-E) and burst forming units (BFU-E) were assayed serially in the peripheral blood, spleen and bone marrow after saponin administration, employing the in vitro methylcellulose culture technique. Animals that had undergone splenectomy prior to saponin administration were also studied. The results demonstrated increases of progenitor cells in the blood and spleen and a simultaneous depletion of such cells in marrow after saponin treatment. The results in splenectomized animals were similar to those observed in non-splenectomized animals after saponin administration. The findings indicate that following saponin administration there is a release of CFU-E and BFU-E from bone marrow into periphery and probably deposition in the spleen, and suggest that myeloid metaplasia in myelofibrosis may result from colonization of extramedullary sites originating from the bone marrow.
采用皂苷诱导的家兔骨髓纤维化和髓外化生模型来研究髓外造血机制。在给予皂苷后，运用体外甲基纤维素培养技术，连续检测外周血、脾脏和骨髓中的造血祖细胞、红系集落形成单位（CFU-E）和爆式集落形成单位（BFU-E）。还对在给予皂苷之前已接受脾切除术的动物进行了研究。结果表明，皂苷处理后，血液和脾脏中的祖细胞增加，同时骨髓中的此类细胞减少。脾切除动物的结果与给予皂苷后未行脾切除动物观察到的结果相似。这些发现表明，给予皂苷后，CFU-E和BFU-E从骨髓释放到外周，可能沉积在脾脏中，并提示骨髓纤维化中的髓外化生可能是由源自骨髓的髓外部位定植所致。
