[菜豆蛋白酶抑制剂反应位点的鉴定]
[Identification of the reactive sites of kidney bean proteinase inhibitor].
作者信息
Mosolov V V, Valueva T A, Malova E L, Kolosova G V, Cheban A N
出版信息
Biokhimiia. 1982 Apr;47(4):561-8.
PMID:7082689
Abstract
The protein proteinase inhibitor from kidney bean (isoinhibitor, pI 4.3) is a double-headed inhibitor with independent reactive sites for trypsin and chymotrypsin. The reactive site of the inhibitor for trypsin is Lys (22)- Ser (23) in the sequense ...-Lys-Ser-Ile-Pro-Ala-Glx-Cys-Arg-..., the reactive site for chymotrypsin is Leu (64)-Ser (65) in the sequence ...-Thr-Leu-Ser-Ile-Pro-Ala-Glx-Cys-Arg-... .
摘要
来自菜豆的蛋白酶抑制剂（同工抑制剂，pI 4.3）是一种双头抑制剂，对胰蛋白酶和胰凝乳蛋白酶具有独立的反应位点。该抑制剂对胰蛋白酶的反应位点在序列...-Lys-Ser-Ile-Pro-Ala-Glx-Cys-Arg-... 中为Lys（22）-Ser（23），对胰凝乳蛋白酶的反应位点在序列...-Thr-Leu-Ser-Ile-Pro-Ala-Glx-Cys-Arg-... 中为Leu（64）-Ser（65）。
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