Tracy P B, Eide L L, Bowie E J, Mann K G
Blood. 1982 Jul;60(1):59-63.
Homogeneous, single-chain human factor V was used to develop a double antibody competition radioimmunoassay to measure factor V concentrations in plasma and platelets. Standard curves were constructed that allow for the detection of as little as 20 ng factor V/ml of plasma. Normal factor V concentrations range from 4 to 14 micrograms/ml of plasma with an average value of 7.0 +/- 2.0 micrograms/ml (n = 64). No correlation was observed between antigen levels and age or sex. The radioimmunoassay data are consistent with factor V clotting assays, providing freshly drawn plasma is used in the bioassay. Radioimmunoassay of washed platelets indicate that 0.63-1.93 microgram of factor V is present per 2.5 X 10(8) platelets (4612-14128 molecules of the factor V platelet). When normalized to individual hematocrits and platelet count, the data indicated that platelets contribute approximately 18%-25% of the factor V found in whole blood. In addition, two individuals with functionally deficient factor V were examined and found to be deficient in both antigen and activity.
使用同源单链人凝血因子V开发了一种双抗体竞争放射免疫测定法，用于测量血浆和血小板中的凝血因子V浓度。构建的标准曲线能够检测低至20 ng凝血因子V/毫升血浆。正常凝血因子V浓度范围为4至14微克/毫升血浆，平均值为7.0±2.0微克/毫升（n = 64）。未观察到抗原水平与年龄或性别之间存在相关性。如果生物测定中使用的是新鲜采集的血浆，放射免疫测定数据与凝血因子V凝血测定结果一致。对洗涤后的血小板进行放射免疫测定表明，每2.5×10⁸个血小板中存在0.63 - 1.93微克凝血因子V（凝血因子V血小板的4612 - 14128个分子）。当根据个体血细胞比容和血小板计数进行标准化时，数据表明血小板贡献了全血中约18% - 25%的凝血因子V。此外，对两名凝血因子V功能缺陷的个体进行了检查，发现他们的抗原和活性均有缺陷。
