S-100 protein synthesis in cultured glioma cell is G1-phase of cell cycle dependent.

The kinetics of S-100 protein synthesis was studied in synchronized cultured glioma (C6) cells. The peak of the synthesis was detected at G1-phase of the cell cycle and apparently was not population density-dependent, since sparsely cultured G1-phase cells synthesized the same proportion of the protein per cell as that of confluent cultures, whereas the total cellular protein synthesis was found steadily increasing throughout the intermitotic phases.