Wei J W, Morris H P, Hickie R A
Cancer Res. 1982 Jul;42(7):2571-4.
Calmodulin contents of normal rat liver, host liver [bearing hepatoma 5123t.c.(h)], regenerating liver, and Morris hepatomas 7800, 5123t.c.(h), and 7794A were determined by phosphodiesterase assay and by radioimmunoassay. The calmodulin levels determined by both assays were significantly increased in three hepatomas when compared to the corresponding values of normal liver. The order of increase in calmodulin content was as follows: normal liver = host liver less than 7794A (slow growth rate) less than 5123t.c.(h) (intermediate growth rate) less than 7800 (fast growth rate). In regenerating liver (24 hr after partial hepatectomy), the calmodulin content was not different from that of normal liver. In good agreement with the literature, the calmodulin values measured by the phosphodiesterase assay were always lower than those determined by radioimmunoassay. Calcium and magnesium contents were measured by atomic absorption spectrophotometry in acid digests of these tissues. Both cation contents were significantly increased in the three hepatomas studied when compared to the corresponding values of normal liver; the extent of increase for calcium content (120 to 240%) was much greater than that for magnesium (30 to 40%). The order of increase for both cations was as follows: normal liver = host liver less than 5123t.c.(h) less than 7794A less than 7800. Therefore, there does not appear to be any correlation between the cation contents and hepatoma growth rates. In regenerating liver, magnesium content was about 14% higher than that of normal liver. In summary, the results indicate that only the increase of calmodulin appears to correlate positively with the growth rate of these tumors. This correlation suggests that calmodulin may be involved in tumor cell growth regulation.
通过磷酸二酯酶测定法和放射免疫测定法，测定了正常大鼠肝脏、宿主肝脏（携带肝癌5123t.c.(h)）、再生肝脏以及莫里斯肝癌7800、5123t.c.(h)和7794A中的钙调蛋白含量。与正常肝脏的相应值相比，两种测定方法所确定的钙调蛋白水平在三种肝癌中均显著升高。钙调蛋白含量增加的顺序如下：正常肝脏 = 宿主肝脏＜7794A（生长速度慢）＜5123t.c.(h)（生长速度中等）＜7800（生长速度快）。在再生肝脏（部分肝切除术后24小时）中，钙调蛋白含量与正常肝脏无异。与文献结果高度一致，通过磷酸二酯酶测定法测得的钙调蛋白值始终低于放射免疫测定法所确定的值。通过原子吸收分光光度法，在这些组织的酸消化液中测量了钙和镁的含量。与正常肝脏的相应值相比，在所研究的三种肝癌中，两种阳离子含量均显著增加；钙含量的增加幅度（120%至240%）远大于镁（30%至40%）。两种阳离子增加的顺序如下：正常肝脏 = 宿主肝脏＜5123t.c.(h)＜7794A＜7800。因此，阳离子含量与肝癌生长速度之间似乎没有任何相关性。在再生肝脏中，镁含量比正常肝脏高约14%。总之，结果表明，只有钙调蛋白的增加似乎与这些肿瘤的生长速度呈正相关。这种相关性表明，钙调蛋白可能参与肿瘤细胞生长调控。
