有袋类和真兽类细胞的融合与杂交。异核体的活性。

Fusion and hybridization of marsupial and eutherian cells. Activity of heterokaryons.

作者信息

Graves J A, Hope R M

出版信息

Aust J Exp Biol Med Sci. 1978 Jun;56(3):341-50. doi: 10.1038/icb.1978.37.

DOI:10.1038/icb.1978.37

Abstract

Marsupial and eutherian nuclei in heterokaryons were shown to synthesize DNA and RNA, apparently at control levels, and heterokaryons were found to contain marsupial hypoxanthine-guanine phosphoribosyltransferase. The two types of nucleus in heterokaryons were also able to undergo synchronous chromosome condensation. our results provide no evidence for suppression of nucleic acid synthesis, gene expression or mitosis in marsupial x eutherian heterokaryons.

摘要

杂种核细胞中的有袋类和真兽类细胞核被证明能合成DNA和RNA，显然处于正常水平，并且发现杂种核细胞含有有袋类次黄嘌呤 - 鸟嘌呤磷酸核糖转移酶。杂种核细胞中的这两种细胞核也能够进行同步染色体浓缩。我们的结果没有提供证据表明有袋类与真兽类杂种核细胞中的核酸合成、基因表达或有丝分裂受到抑制。