Mogilnicka E M, Webb M
Chem Biol Interact. 1982 Jun;40(2):247-56. doi: 10.1016/0009-2797(82)90104-1.
Rats and hamsters, (pre)-treated with copper and cadmium, were used to investigate whether species-differences in renal metallothionein synthesis in response to gold were determined by changes in the kidney concentrations of other metals. The effects of both dietary copper limitation and excess on the renal metabolism of gold also were studied in the rat. In this species, all of the pre-treatments affected the renal concentrations of total and metallothionein-bound copper, but none of them altered either the kidney uptake or thionein-binding of gold. Incorporation of zinc into the metallothionein, which accompanied the binding of gold in this fraction of the kidney, however, was influenced slightly by the pretreatments, In hamsters, pretreatment with cadmium, which increased the concentrations of total and thionein-bound zinc in the kidneys, also did not affect the renal uptake of gold, although it increased significantly the binding of gold to the metallothionein fraction of the renal cytosol. This increased binding of gold also was accompanied by further increases in the zinc and copper contents of the metallothionein; the contents of total and thionein-bound cadmium, however, remained essentially unchanged. Concentrations of copper and zinc in the hamster kidney were not affected significantly by subcutaneous administration of copper alone (five daily doses, each of 3.2 mg Cu/kg body wt.), but were increased when gold was given during the copper-treatment. The concentrations of gold, copper and zinc in the renal metallothionein fraction also were increased under these conditions. From these results it seems that kidney metallothionein synthesis in response to gold may be related to the changes in either the concentration or distribution of zinc, rather than copper.
用铜和镉进行（预）处理的大鼠和仓鼠，被用于研究对金的反应中肾金属硫蛋白合成的物种差异是否由其他金属在肾脏中的浓度变化所决定。还在大鼠中研究了饮食中铜缺乏和过量对金的肾脏代谢的影响。在这个物种中，所有预处理都影响了肾脏中总铜和与金属硫蛋白结合的铜的浓度，但它们都没有改变金在肾脏中的摄取或与硫蛋白的结合。然而，伴随着金在肾脏这一部分中的结合，锌掺入金属硫蛋白中受到预处理的轻微影响。在仓鼠中，用镉进行预处理增加了肾脏中总锌和与硫蛋白结合的锌的浓度，尽管它显著增加了金与肾细胞质中金属硫蛋白部分的结合，但也没有影响金在肾脏中的摄取。金结合的增加还伴随着金属硫蛋白中锌和铜含量的进一步增加；然而，总镉和与硫蛋白结合的镉的含量基本保持不变。单独皮下注射铜（每日5剂，每剂3.2mg铜/千克体重）对仓鼠肾脏中铜和锌的浓度没有显著影响，但在铜处理期间给予金时，其浓度会增加。在这些条件下，肾金属硫蛋白部分中金、铜和锌的浓度也会增加。从这些结果来看，对金的反应中肾脏金属硫蛋白的合成可能与锌的浓度或分布变化有关，而不是与铜有关。
