Klein R A, Keuper H J
Chem Phys Lipids. 1982 Mar;30(1):71-80. doi: 10.1016/0009-3084(82)90008-1.
A modified equilibrium dialysis method is described which is suitable for investigating the binding of fatty acids in the form of aqueous micellar dispersions to proteins. The method uses a permeant chromophore which complexes reversibly with free fatty acid within the dialysis bag. The concentration outside the dialysis bag is determined spectrophotometrically. Binding of oleic acid to bovine serum albumin is given as an example. A simplified analysis of fatty acid binding is given and used to indicate the potential of the method.
本文描述了一种改良的平衡透析方法，该方法适用于研究呈胶束水分散体形式的脂肪酸与蛋白质的结合。该方法使用一种可渗透的发色团，它能与透析袋内的游离脂肪酸可逆地络合。通过分光光度法测定透析袋外的浓度。以油酸与牛血清白蛋白的结合为例进行说明。文中给出了脂肪酸结合的简化分析，并用于表明该方法的潜力。
