Pardini B J, Jones S B, Filkins J P
Circ Shock. 1982;9(2):129-43.
Norepinephrine content (microgram/g) was depressed in hearts and spleens of fasted male Holtzman rats treated intravenously with Salmonella enteritidis lipopolysaccharide (14-17 mg/kg). To investigate the mechanism of norepinephrine depletion during endotoxicosis, in vivo norepinephrine reuptake was evaluated in control and severely shocked rats using the incorporation of 3H-norepinephrine into hearts and spleens. Incorporation of 3H-norepinephrine into spleens of endotoxic rats was reduced 88%, i.e., from a control of 2309 +/- 224 dpm/gm to 270 +/- 69 dpm/gm after endotoxin. In contrast, cardiac tissue incorporation of 3H-norepinephrine was not significantly impaired, i.e., control of 11838 +/- 845 dpm/gm versus severe shock of 17783 +/- 2904 dpm/gm. In vitro analysis of total norepinephrine retained in cardiac and splenic tissue slices incubated with 3H-norepinephrine yielded results consistent with in vivo experiments: Splenic norepinephrine reuptake was significantly decreased on the order of 50% in preparations from endotoxic rats, while myocardial norepinephrine reuptake was the same in both groups. The results indicate that depression of norepinephrine reuptake is a mechanism of norepinephrine depletion in spleens but not hearts of endotoxic rats.
用肠炎沙门氏菌脂多糖（14 - 17毫克/千克）静脉注射处理的禁食雄性霍尔兹曼大鼠，其心脏和脾脏中的去甲肾上腺素含量（微克/克）降低。为了研究内毒素血症期间去甲肾上腺素耗竭的机制，利用将3H - 去甲肾上腺素掺入心脏和脾脏的方法，对对照大鼠和严重休克大鼠的体内去甲肾上腺素再摄取进行了评估。内毒素处理后，3H - 去甲肾上腺素掺入内毒素血症大鼠脾脏的量减少了88%，即从对照的2309±224每分钟衰变数/克降至270±69每分钟衰变数/克。相比之下，心脏组织中3H - 去甲肾上腺素的掺入没有受到显著损害，即对照为11838±845每分钟衰变数/克，严重休克时为17783±2904每分钟衰变数/克。对用3H - 去甲肾上腺素孵育的心脏和脾脏组织切片中保留的总去甲肾上腺素进行的体外分析，得到了与体内实验一致的结果：内毒素血症大鼠制备物中脾脏去甲肾上腺素再摄取显著降低了约50%，而两组中心肌去甲肾上腺素再摄取相同。结果表明，去甲肾上腺素再摄取降低是内毒素血症大鼠脾脏而非心脏中去甲肾上腺素耗竭的一种机制。
