Kelly H, Huggett A, Dawling S
Clin Chem. 1982 Jul;28(7):1478-81.
In this simple and rapid "high-performance" liquid-chromatographic method for determining nitrazepam in plasma, serum, or whole blood, the sample at pH 7.4 is extracted into diethyl ether with an internal standard (prazepam), chromatographed, and detected at 280 nm with a fixed-wavelength ultraviolet detector. A specimen, together with standards and a quality control, can be analyzed in duplicate within 90 min. The limit of sensitivity is 5 micrograms/L (nitrazepam and 7-acetamidonitrazepam) and 50 micrograms/L (7-aminonitrazepam), and no interferents have been found. This method has the advantages of a small sample requirement and complete resolution of nitrazepam and the above-mentioned major metabolites. We have used this method for analysis of therapeutic and overdose concentrations of nitrazepam, and to investigate the stability of the drug in blood.
在这种用于测定血浆、血清或全血中硝西泮的简单快速的“高效”液相色谱法中，将pH 7.4的样品与内标（普拉西泮）一起用乙醚萃取，进行色谱分析，并用固定波长紫外检测器在280 nm处进行检测。一份标本与标准品和质量控制品一起，可在90分钟内重复分析。灵敏度极限为5微克/升（硝西泮和7-乙酰氨基硝西泮）和50微克/升（7-氨基硝西泮），且未发现干扰物。该方法具有样品需求量小以及能完全分离硝西泮和上述主要代谢物的优点。我们已使用该方法分析硝西泮的治疗浓度和过量浓度，并研究该药物在血液中的稳定性。
