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[用一种新型白细胞试纸快速检测尿白细胞。一项多中心合作研究(作者译)]

[Rapid demonstration of urinary leucocytes with a new leucocyte test-strip. A multicentre cooperative study (author's transl)].

出版信息

Dtsch Med Wochenschr. 1982 Jun 4;107(22):853-7.

PMID:7084049
Abstract

In a study in which eight centres from Austria, France, Germany and Switzerland cooperated anew test-strip for urinary leucocytes was compared with chamber counts of uncentrifuged urine, on a total of 3183 urine samples. There was good agreement between the teststrip and reference test as to sensitivity and clinical significance, and no significant difference to the Cytur-Test, which is already commercially available. However, one advantage of the new test is that the reaction time is shortened to one minute, so that it can be used in combination test-strips (Combur-Test), thus providing uniform reading-off time and measurement by reflexion-photometry. There is no interference by ascorbic acid, but albumin and serum proteins in higher concentrations weaken the colour reaction. Urine-preserving formaldehyde-containing additives produce false-positive reactions. Alpha-methyldopa, salicylic acid, beta-hydroxybutyric acid and acetoacetic acid have no influence.

摘要

在一项由来自奥地利、法国、德国和瑞士的八个中心合作开展的研究中,一种新型尿白细胞试纸条与未离心尿液的计数板计数法针对总共3183份尿液样本进行了比较。在敏感性和临床意义方面,试纸条与参考试验之间具有良好的一致性,并且与已上市的Cytur试验相比无显著差异。然而,这种新试验的一个优点是反应时间缩短至1分钟,因此它可以与组合试纸条(Combur-Test)一起使用,从而通过反射光度法提供统一的读数时间和测量。抗坏血酸无干扰,但高浓度的白蛋白和血清蛋白会削弱颜色反应。含甲醛的尿液保存添加剂会产生假阳性反应。α-甲基多巴、水杨酸、β-羟基丁酸和乙酰乙酸无影响。

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