来自牛甲状腺的依赖DNA的RNA聚合酶：催化特性和模板特异性。

DNA-dependent RNA polymerases from bovine thyroid: catalytic properties and template specificities.

Voets R, Lagrou A, Hilderson H J, Van Dessel G, Dierick W

Int J Biochem. 1982;14(5):405-13. doi: 10.1016/0020-711x(82)90026-x.

Abstract

DNA-dependent RNA polymerases I and II have been purified starting from bovine thyroid nuclei yielding a purification factor of 230 for the RNA polymerase I and a purification factor 3212 for RNA polymerase II. RNA polymerase II was further characterized by gel electrophoresis and amino-acid analysis. 2. Kinetics and optimal assay conditions for both RNA polymerases were studied. 3. The template efficiency of a number of DNA preparations was investigated. 4. Rifamycin AF 013 and heparin act as initiation inhibitors. 5. Polyamines were shown to enhance the rate of chain elongation.

摘要

已从牛甲状腺核中纯化出依赖DNA的RNA聚合酶I和II，RNA聚合酶I的纯化因子为230，RNA聚合酶II的纯化因子为3212。通过凝胶电泳和氨基酸分析对RNA聚合酶II进行了进一步表征。2. 研究了两种RNA聚合酶的动力学和最佳测定条件。3. 研究了多种DNA制剂的模板效率。4. 利福霉素AF 013和肝素起起始抑制剂的作用。5. 已表明多胺可提高链延伸速率。