Furuhama K, Takayama S, Onodera T
Nihon Yakurigaku Zasshi. 1982 Mar;79(3):113-21.
The experiment was undertaken to make a discrimination between glomerulonephritis (GN, Masugi-type) and tubular damage (TD, cephaloridine-induced) in rats by means of urinary alkaline phosphatase (AlP). Urinary AlP concentrations were increased 13 times in GN rats amd 8 times in TD rats, in comparison with the corresponding controls. Cellulose acetate plate electrophoresis before and after neuraminidase digestion revealed that urinary AlP from GN rats migrated similarly to serum and intestinal AlP, while the mobility of urinary AlP from TD rats resembled that of the kidney. The sensitivity of urinary AlP from GN to the inhibitors, L-phenylalanine and urea, was almost equivalent to that of intestinal AlP, and the sensitivity of urinary AlP from TD rats was similar to that from kidney AlP. Histochemically, AlP activity in the kidney appeared normal in GN rats, but it was markedly decreased in TD rats. Those results suggest that the urinary AlP from GN rats is identical to serum AlP originating from intestines, i.e., a selective increase in glomerular permeability in disturbed glomerular filtration occurs. On the other hand, urinary AlP from TD rats originated mainly from the kidney due to the disquamation in the proximal convoluted tubular epithelia. Thus the properties of urinary AlP are important for estimating the site of renal damage.
本实验旨在通过尿碱性磷酸酶（AlP）对大鼠肾小球肾炎（GN，马杉吉型）和肾小管损伤（TD，头孢菌素诱导）进行鉴别。与相应对照组相比，GN大鼠的尿AlP浓度增加了13倍，TD大鼠增加了8倍。神经氨酸酶消化前后的醋酸纤维素平板电泳显示，GN大鼠的尿AlP迁移情况与血清和肠AlP相似，而TD大鼠的尿AlP迁移率与肾AlP相似。GN大鼠尿AlP对抑制剂L-苯丙氨酸和尿素的敏感性几乎与肠AlP相当，TD大鼠尿AlP的敏感性与肾AlP相似。组织化学检查显示，GN大鼠肾脏中的AlP活性正常，但TD大鼠的AlP活性明显降低。这些结果表明，GN大鼠的尿AlP与源自肠道的血清AlP相同，即在肾小球滤过紊乱时肾小球通透性选择性增加。另一方面，TD大鼠的尿AlP主要源自肾脏，这是由于近端曲管上皮细胞的脱落。因此，尿AlP的特性对于评估肾损伤部位很重要。
