Tolmasky M E, Staneloni R J, Leloir L F
J Biol Chem. 1982 Jun 25;257(12):6751-7.
The lipid-bound saccharides formed by incubation of uridine diphosphate glucose with a particulate enzyme of Rhizobium meliloti were studied. They behaved like polyprenyl diphosphate saccharides when treated with ammonia or hot phenol, when catalytically hydrogenated, and on DEAE-cellulose chromatography. The saccharide moieties obtained after heating at pH 2 for 10 min at 100 degrees C were separated with a gel filtration column. The following compounds were detected: galactose, glucosyl beta 1-3 galactose (Tolmasky, M. E., Staneloni, R. J., Ugalde, R. A., and Leloir, L. F. (1980) ARch. Biochem. Biophys. 203, 358-364), and some octasaccharides (I). These were compared by paper electrophoresis, thin layer and paper chromatography with an octasaccharide obtained from Alcaligenes faecalis var. myxogenes strain 11 (II). Furthermore, Compounds I and II were compared with the exopolysaccharide of Rhizobium meliloti (III) by partial acid hydrolysis and methylation analysis. The results were consistent with the identity of the repeating unit of Compound III with Compounds I and II except for differences in the substituents (acetyl or succinyl). Studies on the labeling of the lipid-bound saccharides have shown that the sequence is: first, galactose and glucosyl beta 1-3 galactose, then the rest of glucose residues, and finally, the substituents (acetyl and pyruvic acid).
研究了将尿苷二磷酸葡萄糖与苜蓿根瘤菌的一种颗粒酶一起温育形成的脂结合糖。当用氨或热苯酚处理、催化氢化以及在DEAE-纤维素柱层析时，它们的行为类似于聚异戊二烯二磷酸糖。在100℃下于pH 2加热10分钟后得到的糖部分用凝胶过滤柱进行分离。检测到了以下化合物：半乳糖、β1-3葡糖基半乳糖（托尔马斯基，M. E.，斯塔内洛尼，R. J.，乌加尔德，R. A.，以及勒洛伊尔，L. F.（1980年）《生物化学与生物物理学文献》203，358 - 364），以及一些八糖（I）。通过纸电泳、薄层层析和纸层析将这些与从产碱杆菌黏液变种11菌株获得的一种八糖（II）进行了比较。此外，通过部分酸水解和甲基化分析将化合物I和II与苜蓿根瘤菌的胞外多糖（III）进行了比较。结果表明，除了取代基（乙酰基或琥珀酰基）不同外，化合物III的重复单元与化合物I和II相同。对脂结合糖标记的研究表明，顺序是：首先是半乳糖和β1-3葡糖基半乳糖，然后是其余的葡萄糖残基，最后是取代基（乙酰基和丙酮酸）。
