Mori Y, Nagaoka S, Kikuchi T, Tanzawa H
J Biomed Mater Res. 1982 May;16(3):209-18. doi: 10.1002/jbm.820160304.
The change in aggregability of the platelets stored in the storage tube fabricated from a newly developed heparinized hydrophilic polymer (H-RSD) has been studied in comparison with plasticized poly(vinyl chloride) (PVC) which is widely used for blood bags. Rabbit blood was directly withdrawn into the storage tube containing an anticoagulant from the carotid. Then the tube was mechanically sealed with screw cocks and centrifugated to prepare platelet-rich plasma (PRP) in the tube and again mechanically clamped to separate the PRP from the residual precipitate. The PRP was stored in situ in the storage tube at room temperature under agitation. During storage, the change in the aggregability of the PRP induced by adenosine diphosphate (ADP) was studied and the morphological change in the platelets adhered onto the inner surface of the storage tube was observed by scanning electron microscopy. In the H-RSD tube, the aggregability was maintained during two-day storage, while in the PVC tube, the aggregability was completely lost after one-day storage. The scanning electron microscopic studies demonstrated that the reduction in the aggregability of the stored platelets is closely correlated with the morphological deformation of the platelets adhered onto the surface of the storage tube.
与广泛用于血袋的增塑聚氯乙烯（PVC）相比，研究了储存在由新开发的肝素化亲水性聚合物（H-RSD）制成的储存管中的血小板聚集性变化。将兔血直接从颈动脉抽取到含有抗凝剂的储存管中。然后用螺旋旋塞对管子进行机械密封，并离心以在管中制备富血小板血浆（PRP），并再次进行机械夹紧以将PRP与残余沉淀物分离。将PRP在室温下搅拌的条件下原位储存在储存管中。在储存期间，研究了由二磷酸腺苷（ADP）诱导的PRP聚集性变化，并通过扫描电子显微镜观察储存在储存管内表面上的血小板的形态变化。在H-RSD管中，聚集性在两天储存期间得以维持，而在PVC管中，聚集性在一天储存后完全丧失。扫描电子显微镜研究表明，储存血小板聚集性的降低与粘附在储存管表面的血小板的形态变形密切相关。
