Wenthold R J, McGarvey M L
J Neurochem. 1982 Jul;39(1):27-35. doi: 10.1111/j.1471-4159.1982.tb04697.x.
Rapidly transported proteins and glycoproteins in the auditory and optic nerves of the guinea pig were analyzed by electrophoresis and two-dimensional electrofocusing/electrophoresis. Proteins transported in the auditory nerve were analyzed in the cochlear nucleus 3 h after cochlear injection of radioactive precursor, and proteins transported in the optic nerve were analyzed in the superior colliculus 6 h after intraocular injection of radioactive precursor. Two-dimensional analysis showed that several rapidly transported polypeptides were present in one system, but not in the other. By use of [3H]fucose as a precursor or by separating [35S]methionine-labeled polypeptides on immobilized concanavalin A or wheat germ agglutinin, it was shown that most of the proteins transported in only one system are glycoproteins. As previously reported a polypeptide of molecular weight 140,000 was a major labeled species in the auditory nerve. This polypeptide was also found in the optic nerve, but only as a minor species. Two other polypeptides with molecular weights and isoelectric points similar to those of the 140,000 molecular weight polypeptide were present in both systems, but were much more abundant in the optic nerve. The major labeled polypeptide in both systems had a molecular weight of 25,000.
通过电泳和二维电聚焦/电泳分析了豚鼠听觉和视觉神经中快速转运的蛋白质和糖蛋白。在向耳蜗注射放射性前体3小时后，在耳蜗核中分析听觉神经中转运的蛋白质；在眼内注射放射性前体6小时后，在上丘中分析视觉神经中转运的蛋白质。二维分析表明，几个快速转运的多肽存在于一个系统中，而不存在于另一个系统中。通过使用[3H]岩藻糖作为前体，或通过在固定化伴刀豆球蛋白A或麦胚凝集素上分离[35S]甲硫氨酸标记的多肽，结果表明仅在一个系统中转运的大多数蛋白质是糖蛋白。如先前报道，分子量为140,000的一种多肽是听觉神经中主要的标记种类。这种多肽在视觉神经中也有发现，但只是次要种类。另外两种分子量和等电点与分子量为140,000的多肽相似的多肽在两个系统中都存在，但在视觉神经中含量丰富得多。两个系统中的主要标记多肽分子量为25,000。
