Mannweiler K, Hohenberg H, Bohn W, Rutter G
J Microsc. 1982 May;126(Pt 2):145-9. doi: 10.1111/j.1365-2818.1982.tb00363.x.
Due to their high atomic number contrast in transmission electron microscopy, gold particles are ideal markers in surface replicas of cultured cells. The suitability of protein-A-coated gold particles in replica immunocytochemistry for labelling surface antigens is demonstrated using measles virus-infected cells as a model system. Labelled areas can easily be distinguished from unlabelled areas, and even markers positioned in the evaporation shadow of large structures can be accurately identified, which is a prerequisite for an exact quantification and mapping of antigen. In addition, the ultrastructure of labelled areas can still be visualized because of the small size of the marker.
由于金颗粒在透射电子显微镜中具有高原子序数对比度，因此在培养细胞的表面复制品中是理想的标记物。以麻疹病毒感染的细胞作为模型系统，证明了蛋白A包被的金颗粒在复制品免疫细胞化学中用于标记表面抗原的适用性。标记区域很容易与未标记区域区分开来，甚至位于大结构蒸发阴影中的标记物也能被准确识别，这是对抗原进行精确量化和定位的先决条件。此外，由于标记物尺寸小，标记区域的超微结构仍可被观察到。
