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呼肠孤病毒大尺寸类mRNA的核糖体结合位点序列。

Sequences of ribosome binding sites from the large size class of reovirus mRNA.

作者信息

Kozak M

出版信息

J Virol. 1982 May;42(2):467-73. doi: 10.1128/JVI.42.2.467-473.1982.

DOI:10.1128/JVI.42.2.467-473.1982
PMID:7086967
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC256873/
Abstract

Ribosome-protected fragments from two of the large-sized reovirus mRNAs were recovered from sparsomycin-blocked 80S initiation complexes. The sequence of each protected oligonucleotide was determined. The ribosome binding site of each message includes the m7G cap and a centrally positioned AUG codon. An adenine residue occurs three nucleotides upstream from the initiator codon, thus conforming to the pattern [AG]NNAUG observed for nearly all eucaryotic initiation sites.

摘要

从经稀疏霉素阻断的80S起始复合物中回收了来自两种大型呼肠孤病毒mRNA的核糖体保护片段。测定了每个受保护寡核苷酸的序列。每条信息的核糖体结合位点包括m7G帽和位于中心位置的AUG密码子。在起始密码子上游三个核苷酸处出现一个腺嘌呤残基，因此符合几乎所有真核起始位点所观察到的[AG]NNAUG模式。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f131/256873/18b4b8679927/jvirol00158-0127-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f131/256873/db1da1ee2bf2/jvirol00158-0124-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f131/256873/4b1090359c9b/jvirol00158-0126-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f131/256873/18b4b8679927/jvirol00158-0127-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f131/256873/db1da1ee2bf2/jvirol00158-0124-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f131/256873/4b1090359c9b/jvirol00158-0126-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f131/256873/18b4b8679927/jvirol00158-0127-a.jpg

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1
Sequences of ribosome binding sites from the large size class of reovirus mRNA.呼肠孤病毒大尺寸类mRNA的核糖体结合位点序列。
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引用本文的文献

1
Two initiation sites detected in the small s1 species of reovirus mRNA by dipeptide synthesis in vitro.通过体外二肽合成在呼肠孤病毒mRNA的小s1种类中检测到两个起始位点。
Proc Natl Acad Sci U S A. 1984 Feb;81(4):1084-8. doi: 10.1073/pnas.81.4.1084.

本文引用的文献

1
Possible role of flanking nucleotides in recognition of the AUG initiator codon by eukaryotic ribosomes.侧翼核苷酸在真核生物核糖体识别AUG起始密码子中的可能作用。
Nucleic Acids Res. 1981 Oct 24;9(20):5233-52. doi: 10.1093/nar/9.20.5233.
2
Mechanism of mRNA recognition by eukaryotic ribosomes during initiation of protein synthesis.蛋白质合成起始过程中真核生物核糖体对mRNA的识别机制。
Curr Top Microbiol Immunol. 1981;93:81-123. doi: 10.1007/978-3-642-68123-3_5.
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Transcription in vitro by reovirus-associated ribonucleic acid-dependent polymerase.
呼肠孤病毒相关的依赖核糖核酸的聚合酶的体外转录
J Virol. 1970 Jul;6(1):1-11. doi: 10.1128/JVI.6.1.1-11.1970.
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Polypeptide chain initiation: nucleotide sequences of the three ribosomal binding sites in bacteriophage R17 RNA.多肽链起始：噬菌体R17 RNA中三个核糖体结合位点的核苷酸序列
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Nucleotide sequence of a ribonucleic acid transcribed in vitro from lambda phage deoxyribonucleic acid.从λ噬菌体脱氧核糖核酸体外转录的核糖核酸的核苷酸序列。
J Biol Chem. 1971 Aug 25;246(16):5120-39.
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Synthesis and chemical properties of monomers and polymers containing 7-methylguanine and an investigation of their substrate or template properties for bacterial deoxyribonucleic acid or ribonucleic acid polymerases.含7-甲基鸟嘌呤的单体和聚合物的合成及化学性质，以及它们作为细菌脱氧核糖核酸或核糖核酸聚合酶的底物或模板性质的研究。
Biochemistry. 1970 Oct 13;9(21):4141-53. doi: 10.1021/bi00823a017.
7
Sequence and symmetry in ribosome binding sites of bacteriophage f1 RNA.噬菌体f1 RNA核糖体结合位点的序列与对称性
J Mol Biol. 1974 Dec 5;90(2):191-124. doi: 10.1016/0022-2836(74)90368-4.
8
Isolation of intact individual species of single- and double-stranded RNA after fractionation by polyacrylamide gel electrophoresis.通过聚丙烯酰胺凝胶电泳分级分离后，完整的单链和双链RNA单个物种的分离。
Anal Biochem. 1975 May 12;65(1-2):331-45. doi: 10.1016/0003-2697(75)90517-5.
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Synthesis of all the gene products of the reovirus genome in vivo and in vitro.呼肠孤病毒基因组所有基因产物在体内和体外的合成。
Cell. 1975 Feb;4(2):173-80. doi: 10.1016/0092-8674(75)90124-5.
10
Characterization of ribosome-protected fragments from reovirus messenger RNA.呼肠孤病毒信使核糖核酸核糖体保护片段的特性分析
J Biol Chem. 1976 Jul 25;251(14):4259-66.