Kemmler W, Renner R, Zynamon A, Hepp K D
Biochim Biophys Acta. 1978 Oct 18;543(3):349-56. doi: 10.1016/0304-4165(78)90052-1.
Insulin binding experiments were performed with liver plasma membranes from guinea pig, calf and chicken. Bound insulin was separated from free insulin by a simple and rapid centrifugation of membranes through a layer of silicon oil. 125 I-labeled beef insulin was displaced from receptor sites by unlabelled guinea pig, beef and chicken insulin. The receptors of animals with insulins of different biological activity show similar basic characteristics and affinities to the different insulin molecules and thus are not specialised for the interactions with the homologous insulin molecule. The binding capacity of the membranes for beef insulin seems to be inversely related to the affinity of the homologous insulin to the receptor, guinea pig membranes showing the highest and chicken membranes the lowest receptor concentration.
使用豚鼠、小牛和鸡的肝细胞膜进行胰岛素结合实验。通过将膜简单快速地通过一层硅油离心，将结合的胰岛素与游离胰岛素分离。未标记的豚鼠、牛肉和鸡胰岛素将125I标记的牛肉胰岛素从受体位点置换出来。具有不同生物活性胰岛素的动物的受体对不同胰岛素分子表现出相似的基本特征和亲和力，因此并非专门用于与同源胰岛素分子相互作用。膜对牛肉胰岛素的结合能力似乎与同源胰岛素对受体的亲和力呈负相关，豚鼠膜显示出最高的受体浓度，而鸡膜显示出最低的受体浓度。
