Kilpatrick D C, Stirling J L
Biochim Biophys Acta. 1978 Oct 18;543(3):357-63. doi: 10.1016/0304-4165(78)90053-3.
Cells of Distyostelium discoideum representing four developmental stages were auto-analysed for constituent monosaccharides and their compositions compared. Rhamnose, ribose, fucose, glucose, mannose, galactose, glucosamine, galactosamine and an unidentified sugar were recovered after hydrolysis in 2 M HCl for 2 h at 100 degrees C. The relative proportions of the individual sugars were found to vary as a function of development. The largest variations were in the proportions contributed by galactose (from 2% of vegetative cell carbohydrate to 12% of the carbohydrate of fruiting bodies) and galactosamine (present in measurable quantity only in fruiting bodies). Plasma membrane "ghosts" were found to have the same monosaccharide constituents as whole cells, but in different proportions. Mannose contributed over 24% of the total carbohydrate recovered from aggregating cell "ghosts", but only 13% of carbohydrate recovered from "ghosts" prepared from vegetative cells. Galactose was the most abundant sugar recovered from vegetative "ghosts", and was second only to mannose in aggregating "ghosts".
对代表四个发育阶段的盘基网柄菌细胞进行了自身单糖成分分析，并比较了它们的组成。在100℃下于2M盐酸中水解2小时后，回收了鼠李糖、核糖、岩藻糖、葡萄糖、甘露糖、半乳糖、氨基葡萄糖、半乳糖胺和一种未鉴定的糖。发现各个糖类的相对比例随发育而变化。最大的变化在于半乳糖（从营养细胞碳水化合物的2%到子实体碳水化合物的12%）和半乳糖胺（仅在子实体中以可测量的量存在）的比例。发现质膜“空壳”与完整细胞具有相同的单糖成分，但比例不同。甘露糖在从聚集细胞“空壳”中回收的总碳水化合物中占比超过24%，但在从营养细胞制备的“空壳”中回收的碳水化合物中仅占13%。半乳糖是从营养“空壳”中回收的最丰富的糖，在聚集“空壳”中仅次于甘露糖。
