Ferrier W T, Ortmayer H B, Ogasawara F X, Yamamoto R
Poult Sci. 1982 Feb;61(2):379-81. doi: 10.3382/ps.0610379.
Semen from 15 turkeys was pooled and divided into two groups and diluted with glycerated freezing medium. One group contained naturally occurring levels of Mycoplasma meleagridis while the other was inoculated with a 24-hr culture of the organism. Both groups were frozen under identical conditions. Semen was evaluated for motility and life-dead analysis during various stages of cryopreservation. The number of viable mycoplasmas in both groups was determined prior to and at 1-, 2-, and 6-months frozen storage. The experiment was repeated twice. Semen motility and live-dead evaluation showed no apparent trends outside of the normal decline seen during various stages of cryopreservation. There was no significant decline in M. meleagridis levels in either treatment group in either trial when samples were tested at intervals up to 6 months. The naturally infected semen contained approximately 10(3) cfu/ml, while the inoculated semen had 10(5) cfu/ml. It was concluded that viable numbers of M. meleagridis do not substantially decline in turkey semen during cryopreservation and subsequent thawing. Consideration must be given to potential pathogens in turkey semen cryopreserved for long-term storage.
将15只火鸡的精液混合，分成两组，并用甘油冷冻培养基稀释。一组含有自然存在水平的火鸡支原体，另一组接种该生物体的24小时培养物。两组均在相同条件下冷冻。在冷冻保存的各个阶段对精液进行活力评估和死活分析。在冷冻保存前以及冷冻保存1个月、2个月和6个月时，测定两组中活的支原体数量。该实验重复了两次。精液活力和死活评估显示，除了在冷冻保存各个阶段出现的正常下降外，没有明显趋势。在任一试验中，当每隔6个月对样本进行检测时，两个处理组中火鸡支原体水平均未显著下降。自然感染的精液含有约10³ cfu/ml，而接种的精液含有10⁵ cfu/ml。得出的结论是，在冷冻保存和随后解冻过程中，火鸡精液中火鸡支原体的存活数量不会大幅下降。对于长期储存的冷冻保存火鸡精液中的潜在病原体必须予以考虑。
