Matsubara S, Suzuki S, Suzuki H, Kuwabara Y, Okano T
Radiology. 1982 Jul;144(2):295-301. doi: 10.1148/radiology.144.2.7089283.
The effects of contrast material (meglumine iothalamate) on radiation-induced chromosome aberrations were investigated in studies on the lymphocytes of patients who had undergone diagnostic radiography and in in vitro experiments with diagnostic x rays and 60Co gamma rays. Chromosome and chromatid aberrations were found to increase significantly with increasing concentrations of contrast material that were added at irradiation. However, the aberrations were not associated with elevation of the ratio of dicentric and ring chromosomes to the number of cells with unstable chromosome aberrations at the first mitosis. Lymphocytes irradiated in the absence of contrast material did not show an increase in chromosome-type aberrations when the agent was given in increasing concentrations during subsequent incubation, but there were greater numbers of chromatid gaps and breaks. When lymphocytes were exposed to 400 R (103.2 mC/kg) of 60Co gamma rays, the presence of contrast agent did not increase the yield of dicentric and ring chromosomes, but induced a marked delay in cell proliferation, especially in lymphocytes with more heavily damaged chromosomes. In additional examination, the contrast agent itself induced sister chromatid exchanges in lymphocytes.
在对接受诊断性放射照相的患者淋巴细胞进行的研究以及使用诊断性X射线和60钴γ射线进行的体外实验中，研究了造影剂（碘他拉葡胺）对辐射诱导的染色体畸变的影响。发现随着照射时添加的造影剂浓度增加，染色体和染色单体畸变显著增加。然而，在第一次有丝分裂时，这些畸变与双着丝粒和环状染色体与具有不稳定染色体畸变的细胞数量之比的升高无关。在没有造影剂的情况下照射的淋巴细胞，当在随后的孵育过程中给予越来越高浓度的该试剂时，染色体型畸变没有增加，但染色单体间隙和断裂的数量更多。当淋巴细胞暴露于400伦琴（103.2毫库仑/千克）的60钴γ射线时，造影剂的存在并没有增加双着丝粒和环状染色体的产率，但诱导了细胞增殖的明显延迟，特别是在染色体损伤更严重的淋巴细胞中。在进一步的检查中，造影剂本身诱导了淋巴细胞中的姐妹染色单体交换。
