通过麦角新碱在体外对猫软脑膜动脉的抑制作用来证明5-羟色胺受体。
Demonstration of 5-hydroxytryptamine receptors through inhibition by methergoline in cat pial arteries in vitro.
作者信息
Edvinsson L, Hardebo J E
出版信息
Br J Pharmacol. 1978 Oct;64(2):281-3. doi: 10.1111/j.1476-5381.1978.tb17301.x.
Abstract
1 In an attempt to characterize further the nature of the 5-hydroxytryptamine (5-HT)-induced contraction of intracranial vessels, cat's middle cerebral artery was exposed to this amine and the specific 5-HT receptor antagonist, methergoline, under standardized conditions in vitro. Methergoline, in increasing concentrations, produced a parallel shift of the log dose-response curve for 5-HT. 2 The Arunlakshana-Schild plot gave a straight line with a slope of -0.85. The figure corresponding to the pA2 value was 8.80. 3 The findings offer further support for the assumption that the 5-HT-induced intracranial vasoconstriction is mediated by specific 5-HT receptors.
摘要
为了进一步明确5-羟色胺（5-HT）引起的颅内血管收缩的性质，在体外标准化条件下，将猫的大脑中动脉暴露于这种胺类物质和特异性5-HT受体拮抗剂麦角新碱中。随着麦角新碱浓度的增加，5-HT的对数剂量-反应曲线出现平行移动。
阿伦拉克沙纳-席尔德图给出了一条斜率为-0.85的直线。对应pA2值的数字为8.80。
这些发现进一步支持了以下假设，即5-HT引起的颅内血管收缩是由特异性5-HT受体介导的。
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