Acosta D, Mitchell D B, Santone K S, Bock A, Lewis W
Toxicol Lett. 1982 Mar;10(4):385-8. doi: 10.1016/0378-4274(82)90234-x.
Primary cultures of hepatocytes obtained from neonatal Sprague-Dawley rats were grown in arginine-deficient, ornithine-supplemented medium to inhibit fibroblastic overgrowth and to selectively isolate relatively pure cultures of parenchymal hepatocytes. This system of primary hepatocytes was used to study the potential cytotoxicity of ticrynafen by measuring cytoplasmic enzyme leakage, cell viability,, and total protein per culture dish. Hepatic cultures were treated with the drug in concentrations ranging from 10(-3)M to 10(-6)M and for durations from 2 to 8 h. The results of the study indicate that ticrynafen was minimally toxic to the hepatocytes.
从新生斯普拉格-道利大鼠获得的原代肝细胞培养物，在缺乏精氨酸但补充鸟氨酸的培养基中生长，以抑制成纤维细胞过度生长，并选择性地分离出相对纯净的实质肝细胞培养物。该原代肝细胞系统用于通过测量细胞质酶泄漏、细胞活力和每个培养皿中的总蛋白，来研究替克瑞纳芬的潜在细胞毒性。将肝培养物用浓度范围为10⁻³M至10⁻⁶M的药物处理，处理时间为2至8小时。研究结果表明，替克瑞纳芬对肝细胞的毒性极小。
