Kharitonenkov I G, Zakomyrdin Iu A, Elizarova G V
Vopr Virusol. 1982 Mar-Apr;27(2):184-9.
Synthesis of neuraminidase activity in an affinity column with an inhibitor para-amino-phenyloxamine acid (PAPOA), immobilized on sepharose via tripeptide "spacer" (glycyl-glycyl-tyrosine) is described as well as the conditions of PAPOA synthesis. The column has been tested for isolation of neuraminidase types N1 and N2 from various influenza virus strains. The strain specificity of the action of the column in neuraminidase isolation was demonstrated. Neuraminidase free from admixtures has been isolated from the A/PR8/34, A/FM/1/47 and recombinant X-7 strains.
描述了通过三肽“间隔物”（甘氨酰-甘氨酰-酪氨酸）固定在琼脂糖上的抑制剂对氨基苯氧胺酸（PAPOA）在亲和柱中合成神经氨酸酶活性的过程以及PAPOA合成的条件。该柱已用于从各种流感病毒株中分离N1和N2型神经氨酸酶，并证明了该柱在分离神经氨酸酶时作用的菌株特异性。已从A/PR8/34、A/FM/1/47和重组X-7株中分离出无杂质的神经氨酸酶。
