Bell D R, Mullins R J
Am J Physiol. 1982 Jun;242(6):H1044-9. doi: 10.1152/ajpheart.1982.242.6.H1044.
The effect of elevated venous pressure on the extravascular distribution space of albumin and gamma-immunoglobulin G (IgG) in muscle was studied in anesthetized rabbits. In nine rabbits, venous pressure in one leg was increased from 8 to 28 mmHg while the other leg served as a control. Samples of plasma and prenodal muscle lymph were collected for 4 h before taking samples of the gastrocnemius muscle. Lymph was collected from a femoral lymphatic after ligating the popliteal efferent lymphatic. The extracellular and plasma spaces in the tissue samples were measured using 51Cr-labeled ethylenediaminetetraacetic acid (EDTA) and 125I-human serum albumin, respectively. The protein spaces were calculated from measurements of endogenous albumin and IgG concentrations using rocket electroimmunoassay. Albumin and IgG were both excluded from 50% of the interstitial space at lymph concentration. After venous congestion, the interstitial volume was twice that of control. Lymph flow increased from 0.82 +/- 0.18 to 4.44 +/- 0.64 microliters/min. The extravascular mass of albumin and IgG increased. There was no change in the excluded volume fraction for albumin but a decrease in that for IgG. Changes in interstitial protein exclusion in skeletal muscle after pressure elevation may not be important in oncotic buffering of the edema.
在麻醉的兔子身上研究了静脉压升高对肌肉中白蛋白和γ-免疫球蛋白G（IgG）血管外分布空间的影响。在9只兔子中，一条腿的静脉压从8 mmHg升高到28 mmHg，而另一条腿作为对照。在采集腓肠肌样本前4小时收集血浆和结前肌肉淋巴样本。在结扎腘输出淋巴管后从股淋巴管收集淋巴。分别使用51Cr标记的乙二胺四乙酸（EDTA）和125I-人血清白蛋白测量组织样本中的细胞外和血浆空间。使用火箭免疫电泳法根据内源性白蛋白和IgG浓度的测量值计算蛋白质空间。在淋巴浓度下，白蛋白和IgG均被排除在50%的间质空间之外。静脉充血后，间质体积是对照的两倍。淋巴流量从0.82±0.18微升/分钟增加到4.44±0.64微升/分钟。白蛋白和IgG的血管外质量增加。白蛋白的排除体积分数没有变化，但IgG的排除体积分数有所下降。压力升高后骨骼肌间质蛋白排除的变化在水肿的胶体渗透压缓冲中可能并不重要。
