Derks H J, Borrias-van Tongeen V, Bouma A M, Koedam J C
Ann Clin Biochem. 1982 May;19(3):182-6. doi: 10.1177/000456328201900311.
The selective removal from 2-amino-2-methyl-1-propanol solutions of impurities that inhibit alkaline phosphatase activity by means of Amberlite chromatography is described. The effects of buffer concentration, column dimensions, adsorbent type, and zinc sulphate addition were investigated. The method was found to improve greatly the quality of commercial 2-amino-2-methyl-1-propanol solutions and to have several advantages over alternative purification methods.
本文描述了通过安伯莱特色谱法从2-氨基-2-甲基-1-丙醇溶液中选择性去除抑制碱性磷酸酶活性的杂质的方法。研究了缓冲液浓度、柱尺寸、吸附剂类型和硫酸锌添加量的影响。结果发现该方法大大提高了市售2-氨基-2-甲基-1-丙醇溶液的质量，并且相对于其他纯化方法具有多个优点。
