铁转铁蛋白中受保护酪氨酸残基的鉴定。
The identification of protected tyrosine residues in iron-ovotransferrin.
作者信息
Williams J
出版信息
Biochem J. 1982 Mar 1;201(3):647-51. doi: 10.1042/bj2010647.
Abstract
Tetranitromethane reacts with essentially all 21 tyrosine residues of iron-free ovotransferrin. In iron-ovotransferrin, 7 mol of tyrosine/mol of protein are unreactive. Peptides containing the unreactive tyrosine residues were isolated from digests of nitrated iron-ovotransferrin. By comparing the structures of the peptides with the amino acid sequence of ovotransferrin it is found that there are ten protected residues occupying positions 42, 82, 92, 188, 319, 415, 431, 521 and 524 in the polypeptide chain. The problem of identifying the tyrosine residues that form bonds with the metal atoms is discussed.
摘要
四硝基甲烷与去铁卵转铁蛋白的所有21个酪氨酸残基基本上都能发生反应。在铁结合型卵转铁蛋白中，每摩尔蛋白质有7摩尔酪氨酸不发生反应。从硝化的铁结合型卵转铁蛋白的消化产物中分离出了含有这些不反应酪氨酸残基的肽段。通过将这些肽段的结构与卵转铁蛋白的氨基酸序列进行比较，发现多肽链中有10个受保护的残基，位于第42、82、92、188、319、415、431、521和524位。文中讨论了鉴定与金属原子形成键的酪氨酸残基的问题。
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本文引用的文献
1
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J Biol Chem. 1951 Jul;191(1):173-80.
2
INVESTIGATION OF THE IRON AND COPPER COMPLEXES OF AVIAN CONALBUMINS AND HUMAN TRANSFERRINS BY ELECTRON PARAMAGNETIC RESONANCE.通过电子顺磁共振对鸟类伴清蛋白和人类转铁蛋白的铁和铜配合物的研究。
Biochemistry. 1963 Nov-Dec;2:1341-5. doi: 10.1021/bi00906a028.
3
THE STRUCTURE OF A CHYMOTRYPTIC PEPTIDE FROM PSEUDOMONAS CYTOCHROME C-551.来自铜绿假单胞菌细胞色素C-551的一种胰凝乳蛋白酶肽的结构
Biochem J. 1963 Nov;89:379-80. doi: 10.1042/bj0890379.
4
THE AMINO ACID SEQUENCE OF PSEUDOMONAS CYTOCHROME C-551.铜绿假单胞菌细胞色素C-551的氨基酸序列。
Biochem J. 1963 Nov;89(2):349-78. doi: 10.1042/bj0890349.
5
THE SPECIFIC BINDING OF IRON(III) AND COPPER(II) TO TRANSFERRIN AND CONALBUMIN.铁（III）和铜（II）与转铁蛋白和伴清蛋白的特异性结合
Biochim Biophys Acta. 1963 Sep 24;75:203-22. doi: 10.1016/0006-3002(63)90599-7.
6
The preparation and enzymatic hydrolysis of reduced and S-carboxymethylated proteins.还原和S-羧甲基化蛋白质的制备及酶促水解
J Biol Chem. 1963 Feb;238:622-7.
7
[Amino acid determination on paper chromatograms].[纸色谱法测定氨基酸]
Hoppe Seylers Z Physiol Chem. 1957;309(4-6):219-20.
8
Multiple dipping procedures in paper chromatography: a specific test for hydroxy-proline.纸色谱法中的多次浸渍程序：羟脯氨酸的特异性检测
Nature. 1953 Dec 12;172(4389):1100-1. doi: 10.1038/1721100b0.
9
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Annu Rev Biochem. 1980;49:357-93. doi: 10.1146/annurev.bi.49.070180.002041.
10
Periodate inactivation of ovotransferrin and human serum transferrin.
J Biol Chem. 1980 Dec 10;255(23):11429-34.
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