大鼠体内乳酸脱氢酶同工酶M4的内吞作用。用O-(4-重氮-3,5-二[125I]碘苯甲酰基)蔗糖标记酶的实验。
Endocytosis of lactate dehydrogenase isoenzyme M4 in rats in vivo. Experiments with enzyme labelled with O-(4-diazo-3,5-di[125I]iodobenzoyl)sucrose.
作者信息
De Jong A S, Duursma A M, Bouma J M, Gruber M, Brouwer A, Knook D L
出版信息
Biochem J. 1982 Mar 15;202(3):655-60. doi: 10.1042/bj2020655.
Abstract
- Pig lactate dehydrogenase isoenzyme M4 was labelled with O-(4-diazo-3,5-di[125I]iodobenzoyl)sucrose and injected intravenously into rats. Previous work has shown that this label does not influence the clearance of the enzyme (half-life about 26 min) and that it is retained within the lysosomes for several hours after endocytosis and breakdown of the protein [De Jong, Bouma & Gruber (1981) Biochem. J. 198, 45--51]. 2. The distribution of the radioactivity over a large number of tissues was determined 2 h after injection. A high percentage of the injected dose was found in liver (41%), spleen (10%) and bone including marrow (21%). 3. Autoradiography indicated uptake of the enzyme mainly by Kupffer cells of the liver, by spleen macrophages and by bone marrow macrophages. 4. Liver cells were isolated 1 h after injection of the enzyme. Kupffer cells, endothelial cells and parenchymal cells were found to endocytose the enzyme at rates corresponding to 4230, 35 and 25 ml of plasma/day per g of cell protein, respectively. 5. Previous injection of carbon particles greatly reduced the uptake of the enzyme by liver and spleen, but the uptake by bone marrow was not significantly changed.
摘要
- 用O-(4-重氮-3,5-二[¹²⁵I]碘苯甲酰)蔗糖标记猪乳酸脱氢酶同工酶M4，并静脉注射到大鼠体内。先前的研究表明，这种标记不会影响该酶的清除（半衰期约26分钟），并且在蛋白质内吞和分解后，它会在溶酶体内保留数小时[德容、布马和格鲁伯（1981年）《生物化学杂志》198卷，45 - 51页]。2. 注射后2小时测定大量组织中放射性的分布。发现注射剂量的很大一部分存在于肝脏（41%）、脾脏（10%）和包括骨髓在内的骨骼（21%）中。3. 放射自显影显示该酶主要被肝脏的库普弗细胞、脾脏巨噬细胞和骨髓巨噬细胞摄取。4. 注射酶1小时后分离肝细胞。发现库普弗细胞、内皮细胞和实质细胞摄取该酶的速率分别相当于每克细胞蛋白每天4230、35和25毫升血浆。5. 先前注射碳颗粒大大降低了肝脏和脾脏对该酶的摄取，但骨髓的摄取没有明显变化。
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