Farmer J L, Prager M D
Biochem Pharmacol. 1982 Apr 1;31(7):1381-6. doi: 10.1016/0006-2952(82)90032-6.
Dipyridamole (DP, Persantin) was examined for its effects on the proliferation of mitogen-stimulated murine splenocytes and L1210 leukemia cells. In keeping with its reported activity as an inhibitor of nucleoside transport, DP inhibited incorporation by lymphoid cells of labeled thymidine and uridine ino macromolecules. That this inhibition resulted from activities in addition to suppression of nucleoside transport was verified by measured decreases of cellular DNA and viable cell numbers. In addition, protein synthesis was also decreased as indicated by labeled valine incorporation and total protein content of the cells. The rapid accumulation of cAMP in phytohemagglutinin-stimulated splenocytes in the presence of DP may provide an explanation for the anti-proliferative effect of DP on lymphoid cells.
研究了双嘧达莫（DP，潘生丁）对丝裂原刺激的小鼠脾细胞和L1210白血病细胞增殖的影响。与报道的作为核苷转运抑制剂的活性一致，DP抑制淋巴细胞将标记的胸苷和尿苷掺入大分子中。通过测量细胞DNA和活细胞数量的减少，证实这种抑制是由除抑制核苷转运之外的活性引起的。此外，如标记的缬氨酸掺入和细胞总蛋白含量所示，蛋白质合成也减少。在DP存在下，植物血凝素刺激的脾细胞中cAMP的快速积累可能为DP对淋巴细胞的抗增殖作用提供了解释。
