White J F
Biochim Biophys Acta. 1982 May 7;687(2):343-5. doi: 10.1016/0005-2736(82)90567-3.
Isolated segments of jejunum from Amphiuma absorb HCO3+ electrogenically by a process sensitive to acetazolamide. Using a tissue chamber of special design which permits isolation of villus or intervillus epithelium the transepithelial electrical potential (psi ms) of each region was measured. The serosa-negative psi ms generated by the villus epithelium was more negative than that of the intervillus and exhibited greater sensitivity to acetazolamide. Both regions were responsive to other agents which alter epithelial ion transport. The results indicate that intestinal HCO3- absorption is localized predominantly within cells lining the villus epithelium.
从美洲鳗鲡分离出的空肠段通过对乙酰唑胺敏感的过程以电方式吸收HCO₃⁺。使用一种特殊设计的组织室，该组织室允许分离绒毛或绒毛间上皮，测量每个区域的跨上皮电位（ψms）。绒毛上皮产生的浆膜阴性ψms比绒毛间的更负，并且对乙酰唑胺表现出更高的敏感性。两个区域对改变上皮离子转运的其他试剂均有反应。结果表明，肠道HCO₃⁻吸收主要定位于绒毛上皮内衬的细胞内。
