[通过在双相水体系中的分布测定冷冻保护剂对线粒体膜表面性质的影响]
[Effects of cryoprotectors on the properties of the mitochondrial membrane surface as determined by distribution in biphasic aqueous systems].
作者信息
Boĭko N M, Bondarenko V A, Belous A M
出版信息
Biokhimiia. 1982 May;47(5):773-7.
The effects of various cryoprotectors, e. g. dimethylsulfoxide (Me2SO), glycerol, ethylene glycol and polyethylene oxide (PEO, m. w. 400) on the distribution of rat liver mitochondria in a biphasic polymeric PEO 4000-Dextrane 500 system were studied. The type of criss-cross distribution of cryoprotector-treated mitochondria suggests that the isoelectric point for the organelles is shifted towards the acidic values of pH. The effects of these agents decrease in the following order: Me2SO greater PEO-400 greater than glycerol greater than ethylene glycol. The alteration of the distribution coefficient for the mitochondria after their incubation in aqueous polymeric systems in the presence of cryoprotectors is also indicative of a decrease of their surface charge.
研究了各种冷冻保护剂，如二甲基亚砜（Me2SO）、甘油、乙二醇和聚环氧乙烷（PEO，分子量400）对大鼠肝线粒体在双相聚合物PEO 4000-葡聚糖500系统中分布的影响。经冷冻保护剂处理的线粒体的交叉分布类型表明，细胞器的等电点向较低的pH酸性值偏移。这些试剂的作用按以下顺序降低：Me2SO＞PEO-400＞甘油＞乙二醇。在存在冷冻保护剂的情况下，线粒体在水性聚合物系统中孵育后，其分布系数的改变也表明其表面电荷减少。
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