Walz W, Schlue W R
Brain Res. 1982 May 6;239(1):119-38. doi: 10.1016/0006-8993(82)90837-x.
In ion-substitution experiments supplemented by measurements of the membrane conductance, the membrane potential of neuropile glial (NG) cells in the CNS of th medicinal leech has exhibited a dependence of the external concentration of both potassium and chloride. The membrane potential was largely dependent on the external potassium concentration, as may be inferred from the change in potential as the potassium concentration of the bathing solution was changed. The external potassium concentrations had been corrected to allow for the discrepancy between intra- and extraganglionic levels found with ion-selective electrodes. A transient membrane depolarization was recorded when the chloride in the bathing medium was replaced by sulphate or glucuronate. The restoration of the normal membrane potential following the return to chloride-based saline was preceded by a transient hyperpolarization. After transfer to low-chloride solutions, the transient depolarization of the NG cell membrane was followed by a steady-state hyperpolarization. The amplitude of the steady-state hyperpolarization depended on the concentration of chloride in the bathing medium. The membrane conductance decreased in low-chloride solutions.
在补充了膜电导测量的离子替代实验中，药用水蛭中枢神经系统中神经毡胶质（NG）细胞的膜电位表现出对钾离子和氯离子外部浓度的依赖性。膜电位在很大程度上依赖于外部钾离子浓度，这可以从随着浴液中钾离子浓度变化而产生的电位变化推断出来。已对外部钾离子浓度进行了校正，以考虑离子选择性电极所发现的神经节内和神经节外水平之间的差异。当浴液中的氯离子被硫酸盐或葡萄糖醛酸盐替代时，记录到短暂的膜去极化。在恢复到基于氯离子的盐水后，正常膜电位的恢复之前有短暂的超极化。转移到低氯溶液后，NG细胞膜的短暂去极化之后是稳态超极化。稳态超极化的幅度取决于浴液中氯离子的浓度。在低氯溶液中膜电导降低。
