Haldar-Misra C, Pévet P
Cell Tissue Res. 1982;224(1):33-44. doi: 10.1007/BF00217264.
From studies conducted with the pineal organ of the mouse, it was ascertained that for the in vitro investigation of secretory processes (synthesis and release) of proteic/peptidic compound(s), a culture time of 5 to 14 days is optimal. A 5-day organ culture was therefore chosen to study the effects of noradrenaline on these secretory processes. Addition to noradrenaline to the culture medium provokes, in pineal explants of the normal mouse and the "eyeless" mouse, an inhibition of the secretory process, characterized by the formation of granular vesicles. In the hamster and rat, however, opposite results were obtained. Moreover, it appears that noradrenaline, at least in the rat, may also be involved in the regulation of the ependymal-like secretory process. The present results indicate clearly that noradrenaline (thus, the sympathetic innervation) is implicated in the regulation of the production of proteic/peptidic hormonal agents, but that the effect of this neurotransmitter is species-specific. This could explain the numerous contradictory results reported in the literature.
通过对小鼠松果体器官进行的研究确定，对于蛋白质/肽类化合物分泌过程（合成和释放）的体外研究，5至14天的培养时间是最佳的。因此，选择5天的器官培养来研究去甲肾上腺素对这些分泌过程的影响。向培养基中添加去甲肾上腺素会在正常小鼠和“无眼”小鼠的松果体外植体中引发分泌过程的抑制，其特征是形成颗粒状囊泡。然而，在仓鼠和大鼠中获得了相反的结果。此外，似乎去甲肾上腺素，至少在大鼠中，也可能参与室管膜样分泌过程的调节。目前的结果清楚地表明，去甲肾上腺素（因此，交感神经支配）参与蛋白质/肽类激素因子产生的调节，但这种神经递质的作用具有物种特异性。这可以解释文献中报道的众多相互矛盾的结果。
