Lundberg C, Lönnroth J, Nord C E
Infection. 1982;10(2):63-6. doi: 10.1007/BF01816725.
The adherence of Streptococcus pneumoniae to epithelial cells in mucus and to the epithelial lining of the nasopharynx in children was studied with the aid of FITC-labelled anti-sera and Omni-serum. By using acridine orange as a counterstain, other bacteria could be visualized as well. S. pneumoniae was seen to adhere more frequently to desquamated cells in mucus than to squamous cells from the nasopharyngeal wall which were obtained by scraping the dorsal side of the soft palate. No bacteria were found to be attached to the ciliated and metaplastic cells collected from the adenoid surface. Although S. pneumoniae appeared in small numbers in most patients and established microcolonies which were predominantly attached to desquamated cells in mucus, signs of an ecological shift were also observed, with S. pneumoniae constituting the majority of the bacteria present. In such cases many S. pneumoniae could also be seen lying free in mucus.
借助异硫氰酸荧光素标记的抗血清和全血清，研究了肺炎链球菌在儿童黏液中对上皮细胞以及对鼻咽部上皮衬里的黏附情况。通过使用吖啶橙作为复染剂，也可以观察到其他细菌。结果发现，肺炎链球菌在黏液中对脱落细胞的黏附比通过刮取软腭背面获得的鼻咽壁鳞状细胞更频繁。未发现细菌附着于从腺样体表面收集的纤毛细胞和化生细胞。尽管大多数患者体内的肺炎链球菌数量较少，并形成了主要附着于黏液中脱落细胞的微菌落，但也观察到了生态转变的迹象，即肺炎链球菌占所存在细菌的大多数。在这种情况下，还可以看到许多肺炎链球菌游离于黏液中。
