Delacroix D L, Hodgson H J, McPherson A, Dive C, Vaerman J P
J Clin Invest. 1982 Aug;70(2):230-41. doi: 10.1172/jci110610.
In 17 adults, serum, hepatic bile, and saliva samples were analyzed for their sedimentation profile of IgA and secretory component (SC), and for their concentrations of albumin, orosomucoid, transferrin, IgG, IgA, alpha 2-macroglobulin (alpha 2M), IgM, and SC. Polymeric IgA(p-IgA) averaged 13% (50-700 micrograms/ml) of total IgA in serum, 70% (43-88%) in bile, and 93% (74-98%) in saliva. Most of the p-IgA in bile sedimented with SC, which also occurred free (8-44%), and with IgM. In bile, albumin (155-1,485 micrograms/ml) was the predominant protein, followed by IgG (32-480 micrograms/ml), and total IgA (37-209 micrograms/ml). In saliva, p-IgA (72-902 micrograms/ml) predominated, followed by albumin (16-385 micrograms/ml) and IgG (9-178 micrograms/ml). Secretion-to-serum albumin-relative concentration ratios (S/S-ARCR = 1 for albumin) in bile averaged 22 for p-IgA, 1.91 for IgM, 1.28 for monomeric IgA (m-IgA), 0.70 for IgG, and 0.57 for alpha 2M, indicating for p-IgA, IgM, and to a lesser extent for m-IgA, a selective excretion into bile. In saliva, a 16-fold greater selective excretion of p-IgA (mean S/S-ARCR = 354) was found. Labeled m- and p-IgA were injected intravenously into five patients. Specific activities indicated that for p-IgA 50% was serum derived in bile, as compared with 2% in saliva, and to 85% for m-IgA in bile. In the patient with the highest excretion of 125I-p-IgA in bile, only 2.8% of the injected dose was recovered in bile within 24 h after injection. Compared with rats and rabbits, the serum-to-bile transport of p-IgA in humans is much smaller.
对17名成年人的血清、肝胆汁和唾液样本进行了分析，以研究其IgA和分泌成分（SC）的沉降特性，以及白蛋白、类粘蛋白、转铁蛋白、IgG、IgA、α2-巨球蛋白（α2M）、IgM和SC的浓度。血清中聚合IgA（p-IgA）平均占总IgA的13%（50-700微克/毫升），胆汁中占70%（43-88%），唾液中占93%（74-98%）。胆汁中的大多数p-IgA与SC一起沉降，SC也以游离形式存在（8-44%），并与IgM一起沉降。胆汁中，白蛋白（155-1485微克/毫升）是主要蛋白质，其次是IgG（32-480微克/毫升）和总IgA（37-209微克/毫升）。唾液中，p-IgA（72-902微克/毫升）占主导，其次是白蛋白（16-385微克/毫升）和IgG（9-178微克/毫升）。胆汁中分泌与血清白蛋白相对浓度比（S/S-ARCR，白蛋白为1），p-IgA平均为22，IgM为1.91，单体IgA（m-IgA）为1.28，IgG为0.70，α2M为0.57，表明p-IgA、IgM以及程度稍低的m-IgA可选择性排泄到胆汁中。在唾液中，发现p-IgA的选择性排泄增加了16倍（平均S/S-ARCR = 354）。将标记的m-IgA和p-IgA静脉注射到5名患者体内。比活性表明，胆汁中50%的p-IgA来源于血清，而唾液中为2%，胆汁中m-IgA为85%。在胆汁中125I-p-IgA排泄量最高的患者中，注射后24小时内胆汁中仅回收了2.8%的注射剂量。与大鼠和兔子相比，人类中p-IgA的血清到胆汁的转运要少得多。
