Fernald R D
J Comp Neurol. 1982 Apr 20;206(4):379-89. doi: 10.1002/cne.902060406.
The retinal projections of the African cichlid fish, Haplochromis burtoni, have been traced by two different methods. Following unilateral enucleation, a modified Nauta technique was used to demonstrate degenerating axons and terminals. Some degeneration was found after 5 days but optimal survival time was 20-25 days. Orthograde transport of horseradish peroxidase (HRP) into the cut optic nerve also was used to examine retinal fiber distribution in the brain. The optic nerve is completely crossed and gives rise to two major tracts, the tractus opticus dorsomedialis and the tractus opticus ventrolateralis, as well as minor fascicles. Projections were found in the suprachiasmatic nucleus, ventral thalamus, dorsal thalamus, the pretectal complex, and the tectum opticum. The optic tectum is large and laminated and the great majority of the optic fibers terminate there. Degeneration methods revealed projections in the tectum to the stratum opticum, stratum griseum et fibrosum superficiale, and stratum album centrale. HRP staining confirmed these projections and revealed another projection to the stratum griseum centrale.
已通过两种不同方法追踪了非洲丽鱼科鱼类伯氏朴丽鱼的视网膜投射。单侧眼球摘除后，采用改良的瑙塔技术来显示退变的轴突和终末。5天后发现了一些退变，但最佳存活时间为20 - 25天。将辣根过氧化物酶（HRP）正向运输到切断的视神经中也用于检查视网膜纤维在脑中的分布。视神经完全交叉，并形成两条主要束，即背内侧视束和腹外侧视束，以及一些小束。在视交叉上核、腹侧丘脑、背侧丘脑、顶盖前复合体和视顶盖中发现了投射。视顶盖很大且分层，绝大多数视神经纤维在那里终止。退变方法显示视顶盖中投射到视层、浅灰层和纤维层以及中央白层。HRP染色证实了这些投射，并揭示了另一条投射到中央灰层。
