关于一些印度吸虫磷酸酶的组织化学和电泳研究。

Histochemical and electrophoretic studies on phosphatases of some Indian trematodes.

作者信息

Haque M, Siddiqi A H

出版信息

J Helminthol. 1982 Jun;56(2):111-6. doi: 10.1017/s0022149x00034325.

Abstract

The isoenzymes of acid and alkaline phosphatases and their histochemical localization were studied by polyacrylamide disc gel electrophoresis in four species of trematodes: Gigantocotyle explanatum from the liver and Gastrothylax crumenifer from the rumen of water buffalo (Bubalus bubalis) and Echinostoma malayanum and Fasciolopsis buski from the small intestine of the pig (Sus scrofa). Both acid and alkaline phosphatases were present in the tegument, gastrodermis, suckers, testes, ovary, eggs, vitellaria and uterus but alkaline phosphatase activity was demonstrated only in the parenchyma and excretory ducts. Polyacrylamide gel electrophoresis revealed two to four isoenzymes for both acid and alkaline phosphatase.

摘要

通过聚丙烯酰胺圆盘凝胶电泳研究了四种吸虫中酸性和碱性磷酸酶的同工酶及其组织化学定位，这四种吸虫分别是来自水牛（Bubalus bubalis）肝脏的巨大阔盘吸虫（Gigantocotyle explanatum）、来自水牛瘤胃的瘤胃腹袋吸虫（Gastrothylax crumenifer）、来自猪（Sus scrofa）小肠的马来棘口吸虫（Echinostoma malayanum）和布氏姜片吸虫（Fasciolopsis buski）。酸性和碱性磷酸酶均存在于皮层、胃皮层、吸盘、睾丸、卵巢、虫卵、卵黄腺和子宫中，但碱性磷酸酶活性仅在实质组织和排泄管中表现出来。聚丙烯酰胺凝胶电泳显示酸性和碱性磷酸酶均有两到四种同工酶。