Popescu C P, Boscher J, Cribiu E P
J Hered. 1982 Mar-Apr;73(2):147-8.
A technique of cell culture on collagen substrate is proposed for cytogenetic studies on domestic animal embryos. The embryonic cells sampled by biopsy or micromanipulation grow on collagen coated cover slips in Leighton tubes with Ham's F12 medium, supplemented with 20 percent fetal calf serum. This method may be used to study the embryonic karyotype induced by a structural abnormality, as well as the karyotype of domestic animal embryos before transfer.
本文提出了一种在胶原蛋白基质上进行细胞培养的技术，用于家畜胚胎的细胞遗传学研究。通过活检或显微操作采集的胚胎细胞，在含有20%胎牛血清的Ham's F12培养基的Leighton管中，在涂有胶原蛋白的盖玻片上生长。该方法可用于研究由结构异常引起的胚胎核型，以及家畜胚胎移植前的核型。
