Fleming A D, McGaughey R W
J Endocrinol. 1982 Jul;94(1):69-76. doi: 10.1677/joe.0.0940069.
Steroid hormone concentrations (progesterone, oestrone, oestradiol-17 beta) in porcine follicular fluids were measured by radioimmunoassay. In confirmation of several previous studies, as follicular maturation proceeded, the concentrations of all three steroids increased, although the relative amounts of the oestrogens decreased compared to progesterone. Analysis of fluids for steroid-binding components was undertaken using ion-exchange chromatography, gel filtration and steroid-binding assays. Evidence is presented in support of a labile binding component which exhibits high affinity and low capacity for progesterone and little or no affinity for oestadiol. Our findings are discussed in relationship to the known retention of progesterone within preovulatory follicles and to previous reports of steroid-binding components in follicular fluids.
采用放射免疫分析法测定了猪卵泡液中类固醇激素（孕酮、雌酮、雌二醇-17β）的浓度。正如之前多项研究所证实的那样，随着卵泡成熟过程的推进，所有这三种类固醇的浓度均升高，不过与孕酮相比，雌激素的相对含量有所下降。利用离子交换色谱法、凝胶过滤法和类固醇结合分析法对卵泡液中的类固醇结合成分进行了分析。有证据支持存在一种不稳定的结合成分，该成分对孕酮具有高亲和力和低容量，而对雌二醇几乎没有或没有亲和力。我们结合已知的排卵前卵泡内孕酮的潴留情况以及之前关于卵泡液中类固醇结合成分的报道对我们的研究结果进行了讨论。
