Miller S A
J Exp Zool. 1982 Jun 10;221(2):205-11. doi: 10.1002/jez.1402210211.
Morphometric analysis and thymidine autoradiography were used to test the hypothesis that differential growth in the form of proliferative asymmetry is a major contributor to formation of lateral body folds in somatopleure. Chick embryos provided an opportunity to study both spatial and temporal differentials within an individual stage of development. Ectoderm and mesoderm were studied as separate tissues. Thymidine labeling index ranged from 34% to 49% in ectoderm and 43% to 66% in mesoderm and was assumed to reflect rate of proliferation in populations with a 100% proliferation index. Density of cells ranged from ten to 13 cells/1,000 micrometers2 in ectoderm and ten to 15 cells/1,000 micrometers2 in mesoderm, with a mean density of 12 cells/1,000 micrometers2. Cellular proliferation was important in folding, because ectoderm showed highest frequencies of labeling and highest cell density in folding levels. Correlation of proliferative differentials with morphogenesis suggests that proliferation is the form of growth contributing to formation of lateral body folds in 20-somite chick embryos.
以增殖不对称形式存在的差异生长是体壁中侧体褶形成的主要因素。鸡胚为研究个体发育阶段内的空间和时间差异提供了机会。外胚层和中胚层作为独立的组织进行研究。胸腺嘧啶核苷标记指数在外胚层中为34%至49%，在中胚层中为43%至66%，并假定其反映了增殖指数为100%的群体中的增殖速率。细胞密度在外胚层中为每1000平方微米10至13个细胞，在中胚层中为每1000平方微米10至15个细胞，平均密度为每1000平方微米12个细胞。细胞增殖在褶皱形成中很重要，因为外胚层在褶皱水平处显示出最高的标记频率和最高的细胞密度。增殖差异与形态发生的相关性表明，增殖是导致20体节鸡胚侧体褶形成的生长形式。
