通过电子捕获检测气相色谱法测定生物流体中的胍那苄。

Gas chromatographic determination of guanabenz in biological fluids by electron-capture detection.

作者信息

Knowles J A, White G R, Kick C J, Spangler T B, Ruelius H W

出版信息

J Pharm Sci. 1982 Jun;71(6):710-1. doi: 10.1002/jps.2600710627.

Abstract

A sensitive gas chromatographic method for the determination of guanabenz[(2,6-dichlorobenzylidene)amino]guanidine in urine and plasma was developed. The method depends upon the acid hydrolysis of guanabenz to 2,6-dichlorobenzaldehyde, which has strong electron capturing properties and is volatile enough to be eluted from a gas chromatographic column. Concentrations as low as 0.1 ng of guana-benz/ml can be determined and recovery of the drug from urine and plasma samples is 81.8+/- 5.5% (SD). No interferences arising from plasma, urine, or reagents were encountered. Examples of the application of the method are given.

摘要

建立了一种灵敏的气相色谱法，用于测定尿液和血浆中的胍那苄[（2,6 - 二氯亚苄基）氨基]胍。该方法基于胍那苄酸水解生成2,6 - 二氯苯甲醛，2,6 - 二氯苯甲醛具有较强的电子捕获特性，且挥发性足以从气相色谱柱中洗脱出来。能够测定低至0.1 ng/ml的胍那苄浓度，药物从尿液和血浆样品中的回收率为81.8±5.5%（标准差）。未遇到来自血浆、尿液或试剂的干扰。给出了该方法的应用实例。