Inaudi P, Bacigalupo M, Monittola C, Lugaro G, Genazzani A R
J Reprod Fertil. 1982 Jul;65(2):265-73. doi: 10.1530/jrf.0.0650265.
A highly reliable technique for the determination of progesterone in milk has been developed, and its application to pregnancy diagnosis in cows is reported. The method is characterized by an activated charcoal treatment of the samples, extraction of the absorbed progesterone by an organic solvent, followed by RIA. The application of the method to pregnancy diagnosis, 21--23 days after artificial insemination, shows that 5 ng/ml is the most useful value to discriminate between pregnant and non-pregnant animals, with greatest precision on the 21st day, in that 93.5% of pregnant and 100% of non-pregnant animals were correctly diagnosed.
已开发出一种用于测定牛奶中孕酮的高度可靠技术，并报道了其在奶牛妊娠诊断中的应用。该方法的特点是对样品进行活性炭处理，用有机溶剂提取吸附的孕酮，然后进行放射免疫分析。该方法在人工授精后21至23天用于妊娠诊断，结果表明，5纳克/毫升是区分怀孕和未怀孕动物最有用的值，在第21天诊断精度最高，此时93.5%的怀孕动物和100%的未怀孕动物被正确诊断。
