Tiffe H W, Hundeshagen H
J Microsc. 1982 Jun;126(Pt 3):231-5. doi: 10.1111/j.1365-2818.1982.tb00380.x.
Fading and recovery of Af-feulgen stained sperm heads are investigated at 73.5 K. The fast fluorescence signals are measured and stored by two coupled transient recorders. 100% recovery was reached after a dark time of 3 s. This shows that the photodecomposition is mainly caused by change of the probability density of energy level and not by destruction of the chromophore groups. The recovery effect allows measurement of the fluorescence intensity of the same sample more than 50 times. Therefore the unaffected spectrum can be measured directly, provided that between the short-term measurements at constant wavelength an appropriate dark phase has been put into operation.
在73.5K温度下研究了孚尔根染色精子头部的褪色和恢复情况。快速荧光信号由两台耦合的瞬态记录仪进行测量和存储。经过3秒的暗时间后达到了100%的恢复率。这表明光分解主要是由能级概率密度的变化引起的，而不是由发色团的破坏引起的。这种恢复效应使得同一个样品的荧光强度能够被测量50多次。因此，只要在恒定波长下的短期测量之间引入适当的暗相，就可以直接测量未受影响的光谱。
