关于通过单向阴影冷冻蚀刻制剂的电子显微镜确定的小蛋白质颗粒的大小

On the size of small protein particles determined by electron microscopy of unidirectionally shadowed freeze-etched preparations.

作者信息

Schmidt D G, Buchheim W

出版信息

J Microsc. 1982 Jun;126(Pt 3):347-51. doi: 10.1111/j.1365-2818.1982.tb00390.x.

DOI:10.1111/j.1365-2818.1982.tb00390.x

Abstract

The influence exerted by the thickness of the deposited metal layer and the ionic strength of the solution on the apparent size of particles of bovine serum albumin in unidirectionally shadowed freeze-etch preparations of spray-frozen specimens was investigated. It appeared that the size increase due to shadowing is nearly twice the thickness of the deposited metal layer. Apparent particle size was shown to increase linearly with the inverse square root of the ionic strength of the solution. At ionic strength 0.001 the particles appeared about 30% larger than at infinite ionic strength.

摘要

研究了在喷雾冷冻标本的单向阴影冷冻蚀刻制剂中，沉积金属层的厚度和溶液的离子强度对牛血清白蛋白颗粒表观尺寸的影响。结果表明，由于阴影导致的尺寸增加几乎是沉积金属层厚度的两倍。表观颗粒尺寸显示出与溶液离子强度的平方根成反比线性增加。在离子强度为0.001时，颗粒的表观尺寸比在无限离子强度下大30%左右。