Sawada M, Hara N, Ichinose M, Maeno T
J Neurosci Res. 1982;7(2):179-92. doi: 10.1002/jnr.490070209.
The acetylcholine (ACh) responses of anterior aorta muscle cells of Aplysia kurodai were studied by intracellular recording and ACh iontophoresis. Three different types of membrane potential change were observed in response to ACh pulses: a rapid Na+-mediated depolarizing response, a slow, mainly Cl-, in part K+-mediated, hyperpolarizing response, and Na+ (for depolarizing component) and Cl- (for hyperpolarizing component)-mediated biphasic response. The depolarizing response and the depolarizing component of the biphasic response were effectively blocked by hexamethonium or atropine. The hyperpolarizing response and the hyperpolarizing component of the biphasic response were completely blocked by tubocurarine. Low concentrations of ACh inhibited and relaxed the anterior aorta muscle; high concentrations of ACh excited the muscle and initiated contraction. The biphasic responses to ACh are likely due to the presence of more than one ACh receptor type on the same cell membrane of the anterior aorta muscle fiber.
采用细胞内记录法和乙酰胆碱离子导入法，研究了日本黑鳃海兔前主动脉肌细胞对乙酰胆碱（ACh）的反应。在对ACh脉冲的反应中，观察到三种不同类型的膜电位变化：快速的Na⁺介导的去极化反应、缓慢的、主要由Cl⁻介导、部分由K⁺介导的超极化反应，以及Na⁺（去极化成分）和Cl⁻（超极化成分）介导的双相反应。六烃季铵或阿托品可有效阻断去极化反应和双相反应的去极化成分。筒箭毒碱可完全阻断超极化反应和双相反应的超极化成分。低浓度的ACh可抑制并舒张前主动脉肌；高浓度的ACh可兴奋该肌并引发收缩。对ACh的双相反应可能是由于前主动脉肌纤维同一细胞膜上存在不止一种ACh受体类型。
