Emanuel R L, Williams G H
Clin Chem. 1978 Nov;24(11):2042-3.
Collecting blood on ice for renin determination reportedly may produce falsely high results. To assess the probability of this occurring under actual collection conditions, we measured renin activity in duplicate aliquots of plasma from blood samples from 25 hypertensive patients, both supine and upright, and in 10 supine normotensive controls. One aliquot of the blood was collected on ice and processed at 4 degrees C, the other at room temperature. The two aliquots showed no significant differences in renin activity. If anything, values for samples collected at room temperature were higher. Repeat determination on the same specimens stored at--20 degrees C for nine and 12 months revealed no significant changes in results for any samples, although the amount of angiotensin I found in the sample before incubation at 37 degrees C significantly increased. We conclude that it makes little difference at what temperature one collects blood for renin determination, but because of the wide fluctuations in "room" temperature we recommend that blood samples be collected on ice.
据报道，在冰上采集血液用于肾素测定可能会产生假性高结果。为评估在实际采集条件下出现这种情况的可能性，我们对25例高血压患者仰卧位和直立位血样的血浆重复等分样本以及10例仰卧位血压正常对照者的血浆重复等分样本进行了肾素活性测定。一份血液样本在冰上采集并在4℃下处理，另一份在室温下采集并处理。两份等分样本的肾素活性无显著差异。如果有差异的话，室温下采集的样本的值更高。对储存在-20℃下9个月和12个月的相同标本进行重复测定，结果显示任何样本的结果均无显著变化，尽管在37℃孵育前样本中发现的血管紧张素I的量显著增加。我们得出结论，采集血液用于肾素测定时温度的影响不大，但由于“室温”波动较大，我们建议在冰上采集血样。
